Figure 3.

Knockdown of JMJD2B prevented LXRα agonist-induced lipogenic gene expression in HepG2 cells. HepG2 cells were transfected with scramble RNA or JMJD2B siRNA and then treated with T0901317. (A) JMJD2B expression was determined by qPCR and western blotting. (B) Intracellular TG levels were measured using a TG assay kit. (C) mRNA levels of SREBF1, FAS, ACC, and SCD1 were determined by qPCR. (D) Protein levels of LXRα, SREBP1c, and FAS were measured by western blotting. The SREBP1c shown here is precursor form (125 KD). Data are presented as means ± SD from three independent experiments. *** p < 0.001 vs. scramble siRNA.