Table 1.
Scn1b deletion alters excitability of PV + interneurons and pyramidal neurons.
| PV‐Cre/tdTom+ | Layer 5 |
Subiculum |
Layer 6 |
||
|---|---|---|---|---|---|
| Scn1b: | |||||
| Resting Vm | +/+ | −71.2 ± 1.3 | −84.7 ± 1.2 | −78.5 ± 0.5* | −81.7 ± 1.2* |
| (mV) | −/− | −73.0 ± 1.3 | −84.6 ± 1.7 | −75.5 ± 1.0 | −76.8 ± 1.5 |
| Input Resistance | +/+ | 214.2 ± 29.8 | 91.7 ± 5.6 | 135.7 ± 53.5*** | 461.6 ± 36.4**** |
| (MΩ) | −/− | 159.1 ± 26.6 | 101.5 ± 4.9 | 247.0 ± 29.7 | 669.4 ± 37.4 |
| Capacitance | +/+ | 21.7 ± 3.0 | 52.2 ± 3.4 | 32.8 ± 2.3 | 19.5 ± 2.1*** |
| −/− | 22.0 ± 1.4 | 43.2 ± 2.2 | 27.5 ± 1.7 | 14.3 ± 1.0 | |
| Max Firing Rate | +/+ | 85.5 ± 8.1*** | 22.2 ± 1.3 | 26.9 ± 1.4 | 24.8 ± 1.2 |
| −/− | 55.6 ± 3.7 | 19.6 ± 1.0 | 26.6 ± 1.1 | 22.9 ± 1.0 | |
| Depolarization Block | +/+ | 10.5 ± 6.9 | 49.6 ± 12.2 | 22.4 ± 7.0(*) | 63.7 ± 9.3 *** |
| (%) | −/− | 15.7 ± 10.3 | 47.8 ± 12.9 | 46.5 ± 9.2 | 92.8 ± 1.1 |
| 1st AP Peak Voltage | +/+ | 18.5 ± 3.6 | 22.0 ± 1.3 | 30.7 ± 1.2 | 24.8 ± 1.0 |
| (mV) | −/− | 14.8 ± 3.1 | 23.2 ± 1.2 | 27.6 ± 1.5 | 22.9 ± 0.9 |
| AP Train Peak Voltage | +/+ | 14.3 ± 3.4 | 12.1 ± 2.1 | 24.6 ± 1.4** | 18.0 ± 1.3**** |
| (mV) | −/− | 9.1 ± 3.2 | 15.2 ± 1.8 | 18.0 ± 1.9 | 9.1 ± 1.1 |
| 1st AP Peak dv/dt | +/+ | 272.8 ± 23.4 | 176.3 ± 8.4 | 286.7 ± 11.4*** | 170.1 ± 6.5 |
| (mV/ms) | −/− | 209.6 ± 27.2 | 187.3 ± 5.9 | 231.3 ± 13.3 | 158.0 ± 6.5 |
| AP Peak dv/dt Train | +/+ | 223.5 ± 21.4* | 111.0 ± 10.4 | 203.0 ± 14.6*** | 116.2.2 ± 8.2*** |
| (mV/ms) | −/− | 156.6 ± 20.0 | 134.7 ± 8.5 | 131.5 ± 13.3 | 73.4.3 ± 7.3 |
| 1st AP Threshold | +/+ | −60.1 ± 1.5 | −68.7 ± 0.9 | −68.7 ± 0.8 | −65.2 ± 0.7 |
| (mV) | −/− | ‐60.8 ± 2.8 | −68.4 ± 1.0 | −67.8 ± 1.1 | −64.3 ± 0.8 |
| AP Threshold Train | +/+ | −55.5 ± 0.9* | −60.9 ± 0.9 | −60.8 ± 0.9** | −56.5 ± 0.9*** |
| (mV) | −/− | ‐52.9 ± 0.9 | 62.2 ± 1.5 | −56.9 ± 1.1 | −51.6 ± 1.2 |