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. Author manuscript; available in PMC: 2020 Nov 13.
Published in final edited form as: Vasc Med. 2019 Aug 26;24(5):395–404. doi: 10.1177/1358863X19866254

Figure 6.

Figure 6.

p21 KD recovers proliferation and tube formation in DUSP5-deficient ECs under simulated ischemia. (a) Double KD of DUSP5 + p21 in ECs was confirmed by DUSP5 and p21 protein expression in ECs (n = 4, *p < 0.05). (b) DUSP5 protein expression in double KD (n = 4, **p < 0.01). (c) p21 Protein expression in double KD. (d) p21 mRNA expression in double KD (n = 4–6, *p < 0.02, p = 0.014). (e) Representative image of EC tube formation in DUSP5 + p21 double KD. (f) Quantification of tubes/cm2 from experiment in (e) (n = 6, p > 0.05). (g) Recovery of endothelial proliferation in DUSP5 + p21 double KD (Kruskal–Wallis test, n = 8–10, **p < 0.01).

DUSP5, dual specificity phosphatase 5; ECs, endothelial cells; KD, knockdown; NS, not significant; OD, optical density.