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. 2020 Nov 13;32(6):1028–1040.e4. doi: 10.1016/j.cmet.2020.11.006

Figure 2.

Figure 2

ACE2 and TMPRSS2 Protein in Human Islets and Exocrine Tissue of Adult Pancreas

(A–F″) Immunostaining of SARS-CoV-2 cell entry markers ACE2 (red; antibody AF933; A, C, C,′ D, F, and F′) or TMPRSS2 (green; B, C, C″, E, F, and F″) in islet β cells (insulin, blue; C and C′) and islet α cells (glucagon, blue; F and F′) in native pancreatic sections from adult normal donors without diabetes (n = 14 total images analyzed).

(G–L″) Immunostaining of SARS-CoV-2 cell entry markers ACE2 (red; antibody AF933; G, I, I′, J, L, and L′) or TMPRSS2 (green; H, I, I″, K, L, and L″) in islet β cells (insulin, blue; I and I′) and islet α cells (glucagon, blue; L and L′) in native pancreatic sections from adult donors with type 2 diabetes (n = 8 total images analyzed).

(M–R″) Immunostaining of SARS-CoV-2 cell entry markers ACE2 (red; antibody AF933; M, O, O′, P, R, and R′) or TMPRSS2 (green; N, O, O″, Q, R, and R″) in islet β cells (insulin, blue; O and O′) and islet α cells (glucagon, blue; R and R′) in native pancreatic sections from adult donors with type 1 diabetes (n = 4 total images analyzed).

Dotted yellow lines denote islets. Islet (i) and exocrine (e) inset areas are marked by yellow boxes in MERGE column with DAPI counterstain. Pancreatic ducts, identified structurally by rosette pattern of DAPI-labeled nuclei (white), are shown within dotted yellow lines in exocrine INSET column. Human pancreatic donor information is available in Table S2 (A–F″, donor N9; G–L″, donor 2H; M–R″, donor 1C). See also Figures S2–S4. Scale bars, 50 μm (A–R) and 25 μm (insets, C′–R″).