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. 2020 Nov 13;32(6):1028–1040.e4. doi: 10.1016/j.cmet.2020.11.006

Figure 3.

Figure 3

ACE2 Protein Localization with Islet and Exocrine Capillaries in Adult and Juvenile Human Pancreas

(A–C') Representative images of endothelial cells (CD31, white) and ACE2-positive perivascular cells (red; antibody ab15348) in the adult islet microvasculature (n = 14 total images analyzed).

(D and D′) ACE2-positive perivascular cells (red; antibody ab15348) and the extracellular matrix marker collagen-IV (COL4, green) within the vascular basement membrane; DAPI counterstain (blue).

(E–G') Representative images of endothelial cells (CD31, white) and ACE2-positive perivascular cells (red; antibody ab15348) in the exocrine tissue microvasculature (n = 14 total images analyzed).

(H and H′) ACE2-positive perivascular cells (red; antibody ab15348) and the extracellular matrix marker collagen-IV (COL4, green) within the vascular basement membrane; DAPI counterstain (blue).

(I–L′) Representative images of endothelial cells (CD31, white) and ACE2-positive perivascular cells (red; antibody ab15348) in juvenile human islet microvasculature. Islet endocrine cells expressing insulin and glucagon (ENDO, green; L and L′) are shown with DAPI counterstain (blue) (n = 10 total images analyzed).

Yellow arrowheads point to CD31-positive endothelial cells while magenta arrowheads point to perivascular ACE2-positive cells. Inset areas (A′–L′) are marked by yellow boxes in (A)–(L). Human pancreatic donor information is available in Table S2 (A–H′, donor N2; I–L′, donor J4). See also Figure S5. Scale bars, 50 μm (A–L) and 10 μm (insets, A′–L′).