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. 2020 Nov 13;32(6):1028–1040.e4. doi: 10.1016/j.cmet.2020.11.006

Figure 4.

Figure 4

ACE2 Colocalization with Islet Pericytes

(A–C) Representative images of an adult human islet labeled for platelet-derived growth factor receptor β-positive (PDGFRβ, red; A and C) and ACE2 (green; antibody ab15348; B and C) in perivascular cells within a normal donor. DAPI (blue) (n = 20 total images analyzed).

(D) Image of the same islet shown in (A)–(C) with insulin labeling (INS, blue). Inset areas in (A)–(C) are marked by the yellow box.

(E–G′) Representative images of an adult human islet labeled for neuron-glial antigen 2 (NG2, red; E and G), ACE2 (green; antibody ab15348; F and G), and insulin (INS, blue; G) in perivascular cells within a normal donor. DAPI (blue; E, F, and G′). Inset areas in (G′) are marked by yellow boxes in (G) and represent a subset of ACE2-expressing islet pericytes (n = 36 total images analyzed).

(H) Colocalization of ACE2 with NG2- or PDGFRβ-positive cells.

(I) Quantification of ACE2-positive cells expressing pericyte markers PDGFRβ and NG2. Shown are average values obtained for 3 confocal planes per islet for a minimum of 5 islets per each donor of a given age.

Human pancreatic donor information is available in Table S2 (donors HP1754, HP2041, and HP2091). See also Figure S5. Scale bars, 20 μm (A–C), 40 μm (D–G), and 10 μm (G′).