| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse anti-Cytokeratin 19 (1:1000) | Abcam | Cat# ab194399 |
| Rabbit anti-TMPRSS2 (1:400) | Sigma | Cat# HPA035787; RRID: AB_2674782 |
| Mouse anti-Glucagon (1:500-1:1000) | Abcam | Cat# ab10988; RRID: AB_297642 |
| Guinea pig anti-Glucagon (1:500) | LSBio | Cat# LS-C202759-50 |
| Rat anti-C-peptide (1:1000) | DSHB | Cat# GN-ID4; RRID: AB_2255626 |
| Guinea pig anti-Insulin (1:500-1:1000) | Dako | Cat# A0564; RRID: AB_2617169 |
| Rabbit anti-ACE2 (1:500, Figure S2) | Atlas Antibodies | Cat# HPA000288; RRID: AB_1078160 |
| Rabbit anti-ACE2 (1:1000, Figures 1C, 3, 4, S2, S3, and S5) | Abcam | Cat# ab15348; RRID: AB_301861 |
| Goat anti-ACE2 Figures S1) (1:200, Figure S2; 1:400 in Figures 2 and 5) | R&D | Cat# AF933; RRID: AB_355722 |
| Mouse anti-ACE2 (1:33, Figure S2) | R&D | Cat# MAB933; RRID: AB_2223153 |
| Mouse anti-CD31 (1:50) | BD Biosciences | Cat# 550389; RRID: AB_2252087 |
| Goat anti-collagen IV alpha 1 (1:500) | Novus | Cat# NBP1-26549; RRID: AB_1853202 |
| Goat anti-DPP4 (1:200) | R&D | Cat# AF1180; RRID: AB_354651 |
| Mouse anti-NG2 [647] (1:50) | R&D | Cat# FAB2585R |
| Goat anti-PDGFRβ (1:100) | R&D | Cat# AF385; RRID: AB_355339 |
| Donkey anti-mouse-Cy2 (1:500) | Jackson ImmunoResearch | Cat# 715-225-150; RRID: AB_2340826 |
| Donkey anti-mouse-Cy3 (1:500) | Jackson ImmunoResearch | Cat# 715-165-150; RRID: AB_2340813 |
| Donkey anti-mouse-Cy5 (1:250 – 1:500) | Jackson ImmunoResearch | Cat# 715-175-151; RRID: AB_2340820 |
| Donkey anti-rabbit-Cy3 (1:500) | Jackson ImmunoResearch | Cat# 711-165-152; RRID: AB_2307443 |
| Donkey anti-rat-Cy2 (1:500) | Jackson ImmunoResearch | Cat# 712-225-153; RRID: AB_2340674 |
| Donkey anti-guinea pig-Cy2 (1:500) | Jackson ImmunoResearch | Cat# 706-225-148; RRID: AB_2340467 |
| Donkey anti-goat-Cy2 (1:500) | Jackson ImmunoResearch | Cat# 705-225-147; RRID: AB_2307341 |
| Donkey anti-goat-Cy3 (1:500) | Jackson ImmunoResearch | Cat# 705-165-147; RRID: AB_2307351 |
| Donkey anti-guinea pig-Cy5 (1:250-1:500) | Jackson ImmunoResearch | Cat# 706-175-148; RRID: AB_2340462 |
| Normal Donkey Serum | Jackson ImmunoResearch | Cat# 017-000-121; RRID: AB_2337258 |
| Biological Samples | ||
| Human pancreatic islets | Integrated Islet Distribution Program (IIDP) | http://iidp.coh.org; RRID: SCR_014387 |
| Human pancreatic tissue | International Institute for the Advancement of Medicine (IIAM) | http://www.iiam.org; RRID: SCR_016172 |
| Human pancreatic tissue | National Disease Research Interchange (NDRI) | http://ndriresource.org; RRID: SCR_000550 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Propidium Iodide | BD Biosciences | Cat# 556463 |
| Antigen Unmasking Solution, Citrate-Based | Vector Laboratories | Cat# H-3300-250 |
| 1X PBS | Corning | Cat# 46-013-CM |
| Triton X-100 | USB | Cat# 22686 |
| BSA | Sigma | Cat# A6003-25G |
| Human ACE2 Peptide | Abcam | Cat# ab15352 |
| Aqua-Poly/Mount | Polysciences | Cat# 18606-5 |
| 10% Neutral Buffered Formalin | Electron Microscopy Sciences | Cat# 15740-01 |
| 16% paraformaldehyde | Electron Microscopy Sciences | Cat# 15710 |
| O.C.T. compound | Fisher Scientific | Cat# 4585 |
| Critical Commercial Assays | ||
| RNAqueous-Micro Kit | Fisher Scientific (Invitrogen) | Cat# AM1931 |
| High Capacity cDNA Reverse Transcription Kit | Fisher Scientific (Invitrogen) | Cat# 4368814 |
| Deposited Data | ||
| RNA-seq data for FACS purified human alpha and beta cells (Blodgett et al., 2015) | NCBI Gene Expression Omnibus | GEO: GSE67543 |
| RNA-seq data for FACS purified human alpha and beta cells (Arda et al., 2016) | NCBI Gene Expression Omnibus | GEO: GSE57973 |
| Single-cell RNA-seq data for human islets (Baron et al., 2016) | NCBI Gene Expression Omnibus | GEO: GSE84133 |
| Single-cell RNA-seq data for human islets (Camunas-Soler et al., 2020) | NCBI Gene Expression Omnibus | GEO: GSE124742 |
| Single-cell RNA-seq data for human islets (Segerstolpe et al., 2016) | ArrayExpress | ArrayExpress: E-MTAB-5061 |
| Single-cell RNA-seq data for human islets (Human Pancreas Analysis Program [HPAP]) | HPAP Database | https://hpap.pmacs.upenn.edu; RRID: SCR_014393 |
| Software and Algorithms | ||
| FV31S-SW Viewer 2.4.1.198 | Olympus | https://www.olympus-lifescience.com/en/support/downloads/ |
| Seurat 3.1 R package | Butler et al., 2018; Stuart et al., 2019 | N/A |
| Prism for macOS v.8.4.3 | Graphpad Software | http://www.graphpad.com; RRID: SCR_002798 |
| JACoP: Just Another Co-localization Plugin | ImageJ | https://imagej.nih.gov/ij/plugins/track/jacop2.html |
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