Co-expression of Cx40 and renin in the JGA region of developing and healthy postnatal human kidneys: kidneys in the 8th developmental week (a–d); kidneys in the 21–22nd developmental week (e–h); kidneys in the 38th developmental week (i–l), healthy postnatal human kidneys (m–p). Glomeruli (g), afferent arteriole (aa), macula densa (md), juxtaglomerular apparatus (jga), proximal tubule (pt), distal tubule (dt). Cxs or renin expression (arrows), DAPI nuclear stain (c,g,k,o). Merged microphotographs of different Cxs with renin (merge) show their co-expression (arrowheads) in the JGA (d,h,l,p). Double immunofluorescence staining of DAPI nuclear stain with Cx40/renin magnification ×40, scale bar 100 μm (h,p), ×100, scale bar 40 μm (d,l).