Figure 5. The identification of K⁺ channels in setting HCASMC resting membrane potential.

(A–F) The change in the fractional fluorescence of DiBAC₄(3) treated with 10 µM glibenclamide (A), 200 nM penitrem A (B), 1 µM tram34 (C), 200 nM apamin (D), 25 µM BaCl₂ (E), and 10 µM XE991 (F). (G) A summary of percentchange in fractional fluorescence of DiBAC₄(3) after application of K⁺ channel inhibitors: glibenclamide (p < 0.001, paired Student’s (t-test, n = 37 from three independent experiments), penitrem A n = 18 from two independent experiments), tram34 (n = 13 from 1 experiment), apamin (n = 32 from two independent experiments), BaCl₂ (p < 0.001, paired Student’s t-test, n = 26 from two independent experiments), and XE991 (n = 14 from two independent experiments).