REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit monoclonal IgG anti-ACE2 (clone EPR4435(2))	Abcam	Cat# ab108252; RRID: AB_10864415
Rabbit polyclonal anti-ACE2	Abcam	Cat# ab15348; RRID: AB_301861
Mouse monoclonal IgG2A anti-human ACE2 (clone 171606)	R&D Systems	Cat# MAB933; RRID: AB_2223153
Goat polyclonal anti-human ACE2	R&D Systems	Cat# AF933; RRID: AB_355722
Biotinylated goat anti-rabbit IgG	Vector Laboratories	Cat# BA-1000; RRID: AB_2313606
Biotinylated horse anti-mouse IgG (H+L)	Vector Laboratories	Cat# BA-2000; RRID: AB_2313581
Biotinylated rabbit anti-goat IgG (H+L)	Vector Laboratories	Cat# BA-5000; RRID: AB_2336126
Mouse monoclonal IgG1k anti-CD34 (clone QBEnd/10)	Novus Biologicals	Cat# NBP2-32932; RRID: AB_2861355
Rabbit monoclonal IgG anti-insulin (clone EPR17359)	Abcam	Cat# ab181547; RRID: AB_2716761
FLEX guinea pig polyclonal anti-insulin, ready-to-use antibody	Agilent	Cat# IR002; RRID: AB_2800361
Mouse monoclonal IgG1k anti-cytokeratin 19 (clone RCK108)	Agilent	Cat# M0888; RRID: AB_2234418
Goat anti-rabbit IgG (H+L), Alexa Fluor 594	Invitrogen	Cat# A-11037; RRID: AB_2534095
Goat anti-mouse IgG (H+L), Alexa Fluor 488	Invitrogen	Cat# A-11029; RRID: AB_2534088
Goat anti-rabbit IgG (H+L), Alexa Fluor 555	Invitrogen	Cat# A-21428; RRID: AB_2535849
Goat anti-guinea pig IgG (H+L), Alexa Fluor 647	Invitrogen	Cat# A21450; RRID: AB_2735091
Mouse monoclonal IgG1 anti-SARS/SARS-CoV-2 Coronavirus Nucleocapsid protein (clone B46F)	Invitrogen	Cat# MA-1-7404; RRID: AB_1018422
Mouse monoclonal IgG1 anti-glucagon (clone K79bB10)	Abcam	Cat# Ab10988; RRID: AB_297642
Mouse monoclonal IgG1 anti-b-actin (AC-15)	Sigma-Aldrich	Cat# A1978; RRID: AB_476692
Goat anti-guinea pig IgG (H+L), Alexa Fluor 488	Invitrogen	Cat# A-11073; RRID: AB_2534117
Donkey anti-mouse IgG (H+L), Alexa Fluor 488	Invitrogen	Cat# A-21202; RRID: AB_141607
IRDye 800CW Goat anti-rabbit IgG	LI-COR Biosciences	Cat# 926-32211; RRID: AB_621843
IRDye 800CW Goat anti-mouse IgG	LI-COR Biosciences	Cat# 926-32210; RRID: AB_621842
IRDye 800CW Donkey anti-goat IgG	LI-COR Biosciences	Cat# 926-32214; RRID: AB_621846
IRDye 680LT Donkey anti-mouse IgG	LI-COR Biosciences	Cat# 926-68022; RRID: AB_10715072
Biological Samples
Human control organ donor duodenum blocks	nPOD; https://www.jdrfnpod.org/	Table S3
Human control organ donor kidney blocks	nPOD; https://www.jdrfnpod.org/	Table S3
Human control organ donor pancreas blocks	nPOD; https://www.jdrfnpod.org/	Table S3
Human COVID-19 autopsy pancreas blocks	Department of Pathology, Louisiana State University; https://www.medschool.lsuhsc.edu/pathology/	Table S4
Chemicals, Peptides, and Recombinant Proteins
ACE2 peptide	Abcam	Cat# ab198988
Antibody diluent reagent	Life Technologies	Cat# 003118
ImmpactDAB	Vector Laboratories	Cat# SK-4105
VECTASHIELD Antifade Mounting Medium with DAPI	Vector Laboratories	Cat# H-1200
Borg Decloaker RTU	BioCare Medical	Cat# BD1000G1
Background Sniper	BioCare Medical	Cat# BS966L
Protease inhibitor cocktail	Thermo Fisher Scientific	Cat# 78430
Phosphatase inhibitor cocktail	Thermo Fisher Scientific	Cat# 78428
Intercept Blocking Buffer	LI-COR Biosciences	Cat# 927-60001
Intercept Antibody Diluent	LI-COR Biosciences	Cat# 927-65001
ProLong Gold Antifade Mountant	Thermo Fisher Scientific	Cat# P36934
Precision Plus Protein All Blue Prestained Protein Standard	Bio-Rad	Cat# 1610373
Critical Commercial Assays
Avidin/Biotin blocking kit	Vector Laboratories	Cat# SP-2001
Elite standard detection kit	Vector Laboratories	Cat# PK-6100
MACH 2 Double Stain Kit 1 - Anti-Mouse AP/Anti-Rabbit HRP	BioCare Medical	Cat# MRCT523L
MACH 2 Double Stain Kit 2 - Anti-Mouse HRP/ Anti-Rabbit AP	BioCare Medical	Cat# MRCT525L
Betazoid DAB Chromogen Kit	BioCare Medical	Cat# BDB2004L
Warp Red Chromogen Kit	BioCare Medical	Cat# WR806
Ferangi Blue Chromogen Kit	BioCare Medical	Cat# FB813S
RNAscope Multiplex Fluorescent V2 Kit	Advanced Cell Diagnostics	Cat# 323100
TSA Plus Cyanine 3 Fluorescence Kit	Perkin Elmer	Cat# NEL744001KT
TSA Plus Cyanine 5 Fluorescence Kit	Perkin Elmer	Cat# NEL745001KT
Pierce BCA Assay kit	Thermo Fisher Scientific	Cat# 23227
4-20% gradient Mini-PROTEAN TGX Stain-Free gel	Bio-Rad	Cat# 456-8095
Deposited Data
Raw scRNaseq data generated using inDrop	Gene Expression Omnibus Repository; Baron et al., 2016	Table S1; GEO: GSE84133
Raw scRNaseq data generated using Celseq	Gene Expression Omnibus Repository; Grün et al., 2016	Table S1; GEO: GSE81076
Raw scRNaseq data generated using Celseq2	Gene Expression Omnibus Repository; Muraro et al., 2016	Table S1; GEO: GSE85241
Raw scRNaseq data generated using Fluidigm C1	Gene Expression Omnibus Repository; Lawlor et al., 2017	Table S1; GEO: GSE86469
Raw scRNaseq data generated using SMART-Seq2	ArrayExpress; Segerstolpe et al., 2016	Table S1; E-MTAB-5061
Raw and analyzed histology data	This paper	https://aperioeslide.ahc.ufl.edu
Oligonucleotides
RNAscope Probe- Hs-ACE2	Advanced Cell Diagnostics	Cat# 848151
RNAscope Probe- Hs-TMPRSS2-C2	Advanced Cell Diagnostics	Cat# 470341-C2
RNAscope Probe- Hs-TMPRSS4-C2	Advanced Cell Diagnostics	Cat# 565301
RNAscope Probe- Hs-TMPRSS11D-C2	Advanced Cell Diagnostics	Cat# 870841-C4
RNAscope Probe- Hs-CTSL-C2	Advanced Cell Diagnostics	Cat# 858611
RNAscope Probe- Hs-ADAM 17-C2	Advanced Cell Diagnostics	Cat# 468141
Software and Algorithms
Open-source Single-cell R Toolkit, Seurat v.3	Satija Lab; Butler et al., 2018	http://satijalab.org/seurat/
HALO v3.0.311.262	Indica Labs	https://indicalab.com/halo/
Other
Nitrocellulose membranes	LI-COR Biosciences	Cat# 926-31092