| Antibodies | 
| 
 | 
| Rabbit monoclonal IgG anti-ACE2 (clone EPR4435(2)) | Abcam | Cat# ab108252; RRID: AB_10864415 | 
| Rabbit polyclonal anti-ACE2 | Abcam | Cat# ab15348; RRID: AB_301861 | 
| Mouse monoclonal IgG2A anti-human ACE2 (clone 171606) | R&D Systems | Cat# MAB933; RRID: AB_2223153 | 
| Goat polyclonal anti-human ACE2 | R&D Systems | Cat# AF933; RRID: AB_355722 | 
| Biotinylated goat anti-rabbit IgG | Vector Laboratories | Cat# BA-1000; RRID: AB_2313606 | 
| Biotinylated horse anti-mouse IgG (H+L) | Vector Laboratories | Cat# BA-2000; RRID: AB_2313581 | 
| Biotinylated rabbit anti-goat IgG (H+L) | Vector Laboratories | Cat# BA-5000; RRID: AB_2336126 | 
| Mouse monoclonal IgG1k anti-CD34 (clone QBEnd/10) | Novus Biologicals | Cat# NBP2-32932; RRID: AB_2861355 | 
| Rabbit monoclonal IgG anti-insulin (clone EPR17359) | Abcam | Cat# ab181547; RRID: AB_2716761 | 
| FLEX guinea pig polyclonal anti-insulin, ready-to-use antibody | Agilent | Cat# IR002; RRID: AB_2800361 | 
| Mouse monoclonal IgG1k anti-cytokeratin 19 (clone RCK108) | Agilent | Cat# M0888; RRID: AB_2234418 | 
| Goat anti-rabbit IgG (H+L), Alexa Fluor 594 | Invitrogen | Cat# A-11037; RRID: AB_2534095 | 
| Goat anti-mouse IgG (H+L), Alexa Fluor 488 | Invitrogen | Cat# A-11029; RRID: AB_2534088 | 
| Goat anti-rabbit IgG (H+L), Alexa Fluor 555 | Invitrogen | Cat# A-21428; RRID: AB_2535849 | 
| Goat anti-guinea pig IgG (H+L), Alexa Fluor 647 | Invitrogen | Cat# A21450; RRID: AB_2735091 | 
| Mouse monoclonal IgG1 anti-SARS/SARS-CoV-2 Coronavirus Nucleocapsid protein (clone B46F) | Invitrogen | Cat# MA-1-7404; RRID: AB_1018422 | 
| Mouse monoclonal IgG1 anti-glucagon (clone K79bB10) | Abcam | Cat# Ab10988; RRID: AB_297642 | 
| Mouse monoclonal IgG1 anti-b-actin (AC-15) | Sigma-Aldrich | Cat# A1978; RRID: AB_476692 | 
| Goat anti-guinea pig IgG (H+L), Alexa Fluor 488 | Invitrogen | Cat# A-11073; RRID: AB_2534117 | 
| Donkey anti-mouse IgG (H+L), Alexa Fluor 488 | Invitrogen | Cat# A-21202; RRID: AB_141607 | 
| IRDye 800CW Goat anti-rabbit IgG | LI-COR Biosciences | Cat# 926-32211; RRID: AB_621843 | 
| IRDye 800CW Goat anti-mouse IgG | LI-COR Biosciences | Cat# 926-32210; RRID: AB_621842 | 
| IRDye 800CW Donkey anti-goat IgG | LI-COR Biosciences | Cat# 926-32214; RRID: AB_621846 | 
| IRDye 680LT Donkey anti-mouse IgG | LI-COR Biosciences | Cat# 926-68022; RRID: AB_10715072 | 
| 
 | 
| Biological Samples | 
| 
 | 
| Human control organ donor duodenum blocks | nPOD; https://www.jdrfnpod.org/ | Table S3 | 
| Human control organ donor kidney blocks | nPOD; https://www.jdrfnpod.org/ | Table S3 | 
| Human control organ donor pancreas blocks | nPOD; https://www.jdrfnpod.org/ | Table S3 | 
| Human COVID-19 autopsy pancreas blocks | Department of Pathology, Louisiana State University; https://www.medschool.lsuhsc.edu/pathology/ | Table S4 | 
| 
 | 
| Chemicals, Peptides, and Recombinant Proteins | 
| 
 | 
| ACE2 peptide | Abcam | Cat# ab198988 | 
| Antibody diluent reagent | Life Technologies | Cat# 003118 | 
| ImmpactDAB | Vector Laboratories | Cat# SK-4105 | 
| VECTASHIELD Antifade Mounting Medium with DAPI | Vector Laboratories | Cat# H-1200 | 
| Borg Decloaker RTU | BioCare Medical | Cat# BD1000G1 | 
| Background Sniper | BioCare Medical | Cat# BS966L | 
| Protease inhibitor cocktail | Thermo Fisher Scientific | Cat# 78430 | 
| Phosphatase inhibitor cocktail | Thermo Fisher Scientific | Cat# 78428 | 
| Intercept Blocking Buffer | LI-COR Biosciences | Cat# 927-60001 | 
| Intercept Antibody Diluent | LI-COR Biosciences | Cat# 927-65001 | 
| ProLong Gold Antifade Mountant | Thermo Fisher Scientific | Cat# P36934 | 
| Precision Plus Protein All Blue Prestained Protein Standard | Bio-Rad | Cat# 1610373 | 
| 
 | 
| Critical Commercial Assays | 
| 
 | 
| Avidin/Biotin blocking kit | Vector Laboratories | Cat# SP-2001 | 
| Elite standard detection kit | Vector Laboratories | Cat# PK-6100 | 
| MACH 2 Double Stain Kit 1 - Anti-Mouse AP/Anti-Rabbit HRP | BioCare Medical | Cat# MRCT523L | 
| MACH 2 Double Stain Kit 2 - Anti-Mouse HRP/ Anti-Rabbit AP | BioCare Medical | Cat# MRCT525L | 
| Betazoid DAB Chromogen Kit | BioCare Medical | Cat# BDB2004L | 
| Warp Red Chromogen Kit | BioCare Medical | Cat# WR806 | 
| Ferangi Blue Chromogen Kit | BioCare Medical | Cat# FB813S | 
| RNAscope Multiplex Fluorescent V2 Kit | Advanced Cell Diagnostics | Cat# 323100 | 
| TSA Plus Cyanine 3 Fluorescence Kit | Perkin Elmer | Cat# NEL744001KT | 
| TSA Plus Cyanine 5 Fluorescence Kit | Perkin Elmer | Cat# NEL745001KT | 
| Pierce BCA Assay kit | Thermo Fisher Scientific | Cat# 23227 | 
| 4-20% gradient Mini-PROTEAN TGX Stain-Free gel | Bio-Rad | Cat# 456-8095 | 
| 
 | 
| Deposited Data | 
| 
 | 
| Raw scRNaseq data generated using inDrop | Gene Expression Omnibus Repository; Baron et al., 2016 | Table S1; GEO: GSE84133 | 
| Raw scRNaseq data generated using Celseq | Gene Expression Omnibus Repository; Grün et al., 2016 | Table S1; GEO: GSE81076 | 
| Raw scRNaseq data generated using Celseq2 | Gene Expression Omnibus Repository; Muraro et al., 2016 | Table S1; GEO: GSE85241 | 
| Raw scRNaseq data generated using Fluidigm C1 | Gene Expression Omnibus Repository; Lawlor et al., 2017 | Table S1; GEO: GSE86469 | 
| Raw scRNaseq data generated using SMART-Seq2 | ArrayExpress; Segerstolpe et al., 2016 | Table S1; E-MTAB-5061 | 
| Raw and analyzed histology data | This paper | https://aperioeslide.ahc.ufl.edu | 
| 
 | 
| Oligonucleotides | 
| 
 | 
| RNAscope Probe- Hs-ACE2 | Advanced Cell Diagnostics | Cat# 848151 | 
| RNAscope Probe- Hs-TMPRSS2-C2 | Advanced Cell Diagnostics | Cat# 470341-C2 | 
| RNAscope Probe- Hs-TMPRSS4-C2 | Advanced Cell Diagnostics | Cat# 565301 | 
| RNAscope Probe- Hs-TMPRSS11D-C2 | Advanced Cell Diagnostics | Cat# 870841-C4 | 
| RNAscope Probe- Hs-CTSL-C2 | Advanced Cell Diagnostics | Cat# 858611 | 
| RNAscope Probe- Hs-ADAM 17-C2 | Advanced Cell Diagnostics | Cat# 468141 | 
| 
 | 
| Software and Algorithms | 
| 
 | 
| Open-source Single-cell R Toolkit, Seurat v.3 | Satija Lab; Butler et al., 2018 | http://satijalab.org/seurat/ | 
| HALO v3.0.311.262 | Indica Labs | https://indicalab.com/halo/ | 
| 
 | 
| Other | 
| 
 | 
| Nitrocellulose membranes | LI-COR Biosciences | Cat# 926-31092 |