Figure S4.

Timing of Translation-to-Replication Transition, Related to Figure 3

(A) Frequency of multiple infections or phase1 pulses in infected cells. (B) Kaplan-Meier graphs showing durations of infection phase1 (sum of all pulses and breaks; indicated with arrow in Figures 3B and S3C). (C) Quantification of a representative example cell of the number of translating vRNAs over time after 10 μM Rupintrivir treatment. (D) Kaplan Meier graphs of the fraction of uninfected cells remaining after incubation with SunTag-CVB3. Data is corrected for the fraction of cells that were infected before the start of the movies, as indicated by the gap at the start of each graph. (E) Frequency at which indicated number of consecutive time-points without a translating vRNA (gaps) occurred in the analysis of time-lapse movies of STAb cells infected with SunTag-CVB3. (F-K) Kaplan-Meier graphs showing the duration of individual phase1 pulses (F-H) or individual phase1 breaks (I-K). The data plotted in gray (F-K) represents the combination of all data. Datasets with less than six cells were not plotted, but are included in the combined datasets (gray). Data plotted in orange (F-H) is replotted from Figure 3D, for comparison. (L, M) Kaplan-Meier graphs showing the duration of phase3 (J) and phase4 (K). Data plotted in black is replotted from Figure 2J, K, for comparison. The number of experimental repeats and cells analyzed per experiment are listed in Table S1.