Figure S5.

Inhibition of Host Cell Translation, Related to Figure 4

(A) Fluorescence intensity of the labeled methionine analog either in the cytoplasm or in the nucleus after indicated treated measured in single cells. Data are normalized to the mean cytoplasmic intensity. Every dot indicates an experimental repeat; lines indicate mean values. (B) Violin and boxplots of the cytoplasmic fluorescence intensity of the methionine analog in single cells, after indicated treatments. All datasets were normalized to the mean of uninfected (set to 1) and puromycin treated (set to 0) cells. For comparison, the data on uninfected cells in A is replotted from Figures 3B and 3C (in blue). (C) western blots of STAb cells expressing the eIF4G cleavage reporter after indicated incubation times with CVB3. (D) STAb cells expressing the eIF4G cleavage reporter were imaged after infection with SunTag-CVB3. BFP fluorescence intensity differences between mitochondria and cytoplasm over time for infected cells with either successful or unsuccessful replication of the incoming vRNA. The data in black is replotted from Figure 4F. Shaded areas indicate SEM. The number of experimental repeats and cells analyzed per experiment are listed in Table S1.