Figure 7.

Inhibition of sphingosine kinase 1and SIRT1 induces the inactivation of ERK and STAT5 in leukemia cells. (A) TF-1-T315I cells were treated with DMSO, EX527 (5, 10, 20 or 40 µM), SKI-II (20 µM) or SKI-II (20 µM) + EX527 (20 µM) for 24 h and various protein levels were evaluated by western blotting. The protein levels of (B) SIRT1, (C) p-ERK/ERK and (D) p-STAT5/STAT5 in TF-1-T315I cells were quantified. Data are presented as the mean ± standard deviation. ^*P<0.05 and ^***P<0.001 compared with DMSO. SIRT1, sirtuin 1.