Figure 5.

Real time acquisitions of the n-type MOSFET drain-source current I_ds (subtracted by the starting value) in different biosensing experiments, performed in the same conditions (V_ds = 100 mV, V_ref = 400 mV, working buffer: PBS 100 mM, pH 8.0, starting volume: 350 μL). (a–c) Single injections of 20 μL of the working buffer with diluted p53_wt, resulting in a final concentration in the fluid cell of 100 pM, 1 nM and 10 nM. (d) Single injection of 20 μL of the working buffer with diluted HSA (1 nM final concentration). Vertical bars indicate the protein injection time.