Table 2.

The antioxidant activities of different solvent extracts from Myristica fragrans determined with 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), superoxide, and hydroxyl radicals.

Extracting Solvents	DPPH IC50 (μg/mL)	ABTS IC50 (μg/mL)	Superoxide IC50 (μg/mL)	Hydroxyl IC50 (μg/mL)
n-Hexane	126.57 ± 6.23 *	103.05 ± 2.41 *	>400	51.94 ± 0.79 *
Chloroform	167.17 ± 7.13	93.70 ± 5.06 *	>400	82.39 ± 2.62 *
Dichloromethane	96.90 ± 7.68	82.31 ± 2.15 *	>400	88.19 ± 2.09 *
Ethyl acetate	95.12 ± 2.63 *	91.19 ± 0.88 *	>400	55.25 ± 1.25 *
Acetone	65.08 ± 1.44 *	64.35 ± 1.58 *	>400	42.99 ± 0.19 *
Methanol	22.42 ± 0.99 **	34.41 ± 0.78 **	117.66 ± 2.56 *	37.81 ± 1.56 *
Ethanol	39.65 ± 0.83 *	27.68 ± 0.31 **	>400	56.05 ± 2.52 *
BHT a	36.94 ± 0.49 **	11.05 ± 0.26 **	N.A. b	61.51 ± 2.46 *

Results are expressed as half inhibitory concentration (IC₅₀) of each free-radical scavenging activity; ^a Butylated hydroxytoluene (BHT) used as positive control; ^b N.A. indicates not available; * p < 0.05 and ** p < 0.01 compared with the control.