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. 2020 Nov 8;21(21):8371. doi: 10.3390/ijms21218371

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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HWG-35D treatment normalizes both systemic and local immune response induced by IMQ treatment. Gross morphologic features of (A) inguinal lymph node and (B) spleen. (C) Total cell numbers in inguinal lymph nodes were analyzed. (D) Spleen weights and (E) total cell numbers of the spleen were examined. (F) Serum IL-17A levels were investigated using ELISA. The mRNA levels of (G) IL-17A, (H) IL-17F, (I) keratin 6 (K6), and (J) keratin 16 (K16) were analyzed using real-time PCR. Data are mean ± S.E.M. values (n = 5). * p < 0.05, ** p < 0.01, *** p < 0.001.