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. 2020 Nov 7;21(21):8361. doi: 10.3390/ijms21218361

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of 1 h PFF on paxillin expression and distribution in MC3T3-E1 osteoblasts. (A) mRNA expression of PXNA. (B) mRNA expression of PXNB. (C) 2D images of control cells stained for paxillin (red) and DAPI for the nucleus (blue). (D) 2D images of 1 h PFF-treated cells stained for-paxillin and nucleus. Bar = 40 µm. (E) Fluorescence intensity of paxillin in control and PFF-treated cells. (F) Paxillin number in control and PFF-treated cells. (G) Paxillin area in control and PFF-treated cells. (H) Paxillin average size in control and PFF-treated cells. * p < 0.05. The contrast in Figure 4C and D were enhanced for clarity. CON, control; PFF, pulsating fluid flow.