Figure 3.
Autophagy activation in SH-SY5Y cells with apelin-13 treatment. (A–C) SH-SY5Y cells were treated with apelin-13 (10−11 mol/L ~ 10−9 mol/L) for 24 h. Western blot was applied to detect the expression of light chain 3B (LC3B)-II/LC3B-I and p62. The ratio of LC3B-II/LC3B-I was increased dose-dependently with apelin-13 (10−10 mol/L ~ 10−9 mol/L) treatment, while the expression of p62 was reduced as compared with the control. (D–G) SH-SY5Y cells were treated with apelin-13 or co-treated with apelin-13 and CQ (10 μM). The ratio of LC3B-II/LC3B-I and the expression of p62 were increased in the presence of CQ, and further increased with apelin-13 and CQ in SH-SY5Y cells. Data are presented as mean ± SEM of three replications. * p < 0.05; ** p < 0.01 compared with the control; ## p < 0.01 compared with the apelin-13 group.