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. 2020 Nov 8;21(21):8376. doi: 10.3390/ijms21218376

Figure 4.

Figure 4

Effects of apelin-13 on rotenone-induced autophagy impairment in SH-SY5Y cells. (A) Fluorescent images were captured and processed. Cells in the control and rotenone groups exhibited a diffuse distribution of LC3BⅡ. The number of LC3BⅡpuncta increased in the presence of apelin-13, with or without rotenone treatment. Bar = 20 μm. (B) Statistical analysis of LC3 puncta/cell. Data are presented as mean ± SEM of three replications. ** p < 0.01 compared with the control; ## p < 0.01 compared with the rotenone group. (C–E) Western blot was applied to detect the levels of LC3B-II/LC3B-I and p62. Apelin-13 rescued the ratio of LC3B-II/LC3B-I and blocked the expression of p62 in the rotenone-treated SH-SY5Y cells. (F,G) Western blot was applied to detect the expression of α-synuclein. Rotenone treatment led to a dramatic increase in α-synuclein levels in SH-SY5Y cells; however, apelin-13 pretreatment significantly reduced this increase. Data are presented as mean ± SEM of three replications. * p < 0.05; ** p < 0.01 compared with the control; ## p < 0.01 compared with the rotenone group.