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. 2020 Nov 8;21(21):8376. doi: 10.3390/ijms21218376

Figure 11.

Figure 11

Effects of APJ siRNA on autophagy in SH-SY5Y cells with rotenone and apelin-13 treatment. (A–C) SH-SY5Y cells were treated with apelin-13 or co-treated with apelin-13 and APJ siRNA. Western blot was applied to detect the phosphorylation of LC3B-II/LC3B-I and p62. APJ siRNA abolished the increased LC3B-II/LC3B-I ratio induced by apelin-13, and restored p62 levels in the SH-SY5Y cells. Data are presented as mean ± SEM of three replications. * p < 0.05; ** p < 0.01 compared with the control; # p < 0.05; ## p < 0.01 compared with the apelin-13 group. (D–G) SH-SY5Y cells were exposed to rotenone with or without apelin-13, or with APJ siRNA pretreatment prior to rotenone and apelin-13. Western blot was applied to detect the expression of LC3B-II/LC3B-I, p62, and α-synuclein. Apelin-13 significantly increased the ratio of LC3B-II/LC3B-I, and reduced the expression of p62 and α-synuclein in rotenone-treated SH-SY5Y cells; APJ siRNA pretreatment abolished these effects. Data are presented as mean ± SEM of three replications. * p < 0.05; ** p < 0.01 compared with the control; ## p < 0.01 compared with the rotenone group; △ p < 0.05; △△ p < 0.01 compared with the Apelin-13+Rotenone group.