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. 2020 Nov;20(4):459–470. doi: 10.17305/bjbms.2019.4503

FIGURE 5.

FIGURE 5

ZFP36L1 overexpression reversed miR-129-5p-mediated proliferation inhibition in GBM. (A) MiR-129-5p-overexpressing LN229 and A172 cells were transfected with pcDNA3.1/+ vector or pcDNA3.1/ZFP36L1, and the expression of ZFP36L1 was determined by quantitative reverse transcription PCR and Western blotting analysis. The mRNA (left panel) and protein (right panel) levels of ZFP36L1 were significantly increased after the transfection. (B) Following the transfection, the growth rates of LN229 and A178 cells were determined by CCK-8 assay. The absorbance was recorded at 450 nm for every 24 h. ZFP36L1 overexpression restored the proliferation inhibition by miR-129-5p in LN229 and A178 cells. ZFP36L1: ZFP36 ring finger protein-like 1; GBM: glioblastoma multiforme. Differences were considered statistically significant at *p < 0.05.