Figure 1.

Analysis of carbonic anhydrase IX (CA IX) expression in triple-negative breast cancer (TNBC) sample patients and cell lines. (A) In silico analysis of mRNA CA IX expression was performed on two different datasets: GSE16391 which includes 55 non-triple-negative breast primary tumors and GSE76124 which includes 198 TNBC tumors from MD Anderson Cancer Center. The box plot represents a comparison of the CA IX expression between non-TNBC and TNBC tumor samples * p < 0.0001. (B) MDA-MB-231 and BT-549 cells were transfected for 48 h with CA IX-specific siRNAs (100 nM) or non-targeting siRNAs (siRNA Scr) (100 nM), used as negative control, in 1% O₂. A control was performed in 21% O₂. CA IX protein levels were analyzed using Western blot analysis. Actin was used as loading control. Representative data from one of three experiments are shown.