Figure 2.

Targeting CA IX reduces TNBC vasculogenic mimicry. BT-549 (A) and MDA-MB-231 (B) were grown in normoxia (21% O₂) or hypoxia (1% O₂) conditions on the surface of Matrigel and vascular loops were analyzed. TNBC cell grown in 1% O₂ were transfected with siRNA CA IX (100 nM) and siRNA Scr (100 nM) for 48 h or treated with RC44 (100 µM) and seeded into 24-well plates pre-coated with 80 µL/well Matrigel. Representative images were taken at 10× and the average of the number of complete loops was calculated from 3–5 random fields by a macro made with ImageJ software. Bars depict mean ± SD of three independent experiments. *** p < 0.0001; ** p < 0.001.