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. 2020 Nov 9;21(21):8405. doi: 10.3390/ijms21218405

Figure 3.

Figure 3

(A) Chemical Structure of CA inhibitor RC44. (B) Effect of CA inhibitor RC44 on TNBC cell proliferation. Cell viability (MTS assay) of BT-549 and MDA-MB-231 (50 × 103 cells/well), treated with different concentrations of RC44 (from 5 µM to 250 µM) for 72 h in hypoxic conditions (1% O2). All the data are expressed as percentage of viable cells, considering the untreated control cells as 100%. (C) Effect of RC44 on extracellular pH (pHe) in hypoxia. BT-549 and MDA-MB-231 were grown under normoxic (21% O2) and hypoxic conditions (1% O2) for 72 h and treated with RC44 (100 μM) when grown in hypoxia. Bars depict mean ± SD of three independent experiments. (** p < 0.001; * p < 0.01).