Figure 5.

Effect of CA inhibitor RC44 on cell wound healing ability. BT-549 (A) and MDA-MB-231 (B) were grown in normoxia (21%O₂) or hypoxia (1%O₂) conditions and were scratched with pipette tips to create wounds. After removal of detached cells, medium containing 1% FBS, 10% FBS and RC44 (100 µM) were added to cells. Each scratch area was photographed at 0, 24 h. The distance between the edges of the scratch was measured by ImageJ, the average distance was quantified and the extent of wound closure was determined as follows: Wound closure (%) = 1 − (wound width tx/wound width t0) × 100. Bars depict mean ±SD of three independent experiments. *** p < 0.0001.