Figure 6.

Blocking CA IX inhibits TNBC invasiveness. Boyden chamber coated with Matrigel to mimic ECM was used for cell invasion assay. BT-549 (A) and MDA-MB-231 (B) were grown in normoxia (21% O₂) or hypoxia (1% O₂) conditions and their invasion ability was analyzed. Medium containing 1% FBS or 10% FBS was added to the lower chamber as chemoattractant. The results are expressed as the percentage of invasive cells considering the untreated control sample (1% FBS) as 100%. TNBC cells grown in 1% O₂ were transfected with siRNA CA IX (100 nM) and siRNA Scr (100 nM) or treated with RC44 (100 µM) and seeded in the upper chamber. Medium containing 10% FBS was added to the lower chamber as chemoattractant. The results are expressed as the percentage of invasive cells considering the untreated control sample (10% FBS) as 100%. Bars depict mean ±SD of three independent experiments. *** p < 0.0001; ** p < 0.001.