Table 1.
Characterization of macrophages regarding cell area, number of lysosomes/cell and area of lysosome according to LysoID staining in the different solvent. Means ± SEM are listed and significant differences to AD exposed cells indicated by asterisk. Abbreviation: DMSO, dimethylsulfoxide; AD, distilled water; GM-MDM, M1 macrophages.
| Macrophages | Cell Area (DMSO, µm2) | Cell Area (AD, µm2) | Lysosome Number (DMSO) | Lysosome Number (AD) | Lysosome Area (DMSO, µm2) | Lysosome Area (AD, µm2) |
|---|---|---|---|---|---|---|
| DMBM-2 | 253 ± 6 * | 229 ± 2.4 | 30 ± 0.5 | 30 ± 0.4 | 3.5 ± 0.014 | 3.3 ± 0.009 |
| J774A.1 | 183 ± 1.8 * | 172 ± 1.6 | 24 ± 0.4 | 23 ± 0.4 | 1.5 ± 0.008 | 1.4 ± 0.006 |
| RAW264.7 | 131 ± 1.6 * | 120 ± 1.7 | 13 ± 0.2 | 14 ± 0.2 | 1.1 ± 0.003 | 1.1 ± 0.004 |
| THP-1 | 208 ± 3.8 | 215 ± 3.3 | 13 ± 0.4 | 14 ± 0.5 | 1.5 ± 0.01 | 1.5 ± 0.01 |
| GM-MDM | 241 ± 4.5 | 238 ± 4.0 | 11 ± 0.2 | 10 ± 0.2 | 2.1 ± 0.01 | 2.2 ± 0.01 |