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. 2020 Nov 12;10:19694. doi: 10.1038/s41598-020-76684-0

Figure 1.

Figure 1

Characterization of DPSC cultures. (A) Representative phase-contrast microscopic image of DPSC at passage 3 displays a spindle-like morphology with relatively thin processes extending from the cell body. (B) Relative expression profiles of Nanog, Sox2, Nestin, CD29, CD90, CD105, CD14 and CD45 in DPSC in relation to the expression of a housekeeping gene, GAPDH. (C) Differentiation along adipogenic lineage (Oil red O), (D) Osteogenic lineage (alizarin red). Scale bar: 100 μm. (E) SqRT-PCR confirming the osteogenic and adipogenic differentiation of DPSC by the expression of mineralization markers; alkaline-phosphatase (ALP), osteopontin (OPN) and adipogenic marker; lipoprotein lipase (LPL) in the three tested groups; undifferentiated control, osteogenic and adipogenic. GAPDH is a housekeeping control.