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. 2020 Nov 12;10:19694. doi: 10.1038/s41598-020-76684-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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DPSC promote cell survival and decrease cell death. After 4 days exposure period of TGNC to; serum-free DMEM/F12 (control), 50% DPSC-CM, 50 ng/ml NGF or co-cultured with DPSC, the number of viable cells was significantly higher in DPSC-CM treated cultures. (A) Laser confocal images of live/dead TGNC assay, scale bar: 100 µm. (B) Quantitative live/dead assay results obtained from Tecan fluorescence plate reader. (C) Total viable cell number is confirmed by MTT assay, serum-free control was kept at 100% and all other groups were relative to it. Data are presented as mean percentage ± SEM, from 3 independent experiments with 15 replicates for each group/experiment.