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. 2020 Nov 12;10:19614. doi: 10.1038/s41598-020-76256-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Scheme of the competition assay. Bacteria were isolated from the sponge specimens. As example a blue and green coloured sponge are shown. All axenic cultures derived from one sponge were tested against all the others from one sponge. Therefore, bacteria were cultured and agar plugs were transferred to agar plates inoculated with the test strain. Best competitors (inhibiting most of the test strains, which was observed by inhibition zone) were selected and tested against best competitors derived from other sponges.