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. 2020 Nov 12;10:19644. doi: 10.1038/s41598-020-76883-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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ZO-1 expression in human pulmonary diseases related to inflammation. (a) Three different tissues from normal, hyperplasia, and inflammation groups were used. Lung sections were processed with anti-ZO-1 antibody, stained with Deep-Red, and images were obtained for each core. Five separate images of each section were given a score (1– 7) by three independent scientists. (b) The expression density was used to assess stained differences between cells. *p < 0.05 compared with normal. All data shown are representative of three independent experiments.