Table 3.

miRNA expression differences between ME/CFS group and healthy controls by RT-qPCR in the replication phase.

miRNA	T0	T90
	Fold difference	Fold difference
hsa-miR-28-5p	2.24 ± 0.47*	2.65 ± 0.97
hsa-miR-29a-3p	1.71 ± 0.33	1.73 ± 0.33
hsa-miR-127-3p	4.27 ± 0.78**	2.86 ± 0.47
hsa-miR-140-5p	3.08 ± 0.61**	2.00 ± 0.32
hsa-miR-150-5p	3.37 ± 1.05	8.61 ± 2.53*
hsa-miR-181b-5p	1.56 ± 0.29	1.72 ± 0.40
hsa-miR-374b-5p	2.70 ± 0.56*	2.00 ± 0.35
hsa-miR-486-5p	1.68 ± 0.20	3.02 ± 0.71*
hsa-miR-3620-3p	1.36 ± 0.15	3.30 ± 0.76*
hsa-miR-4433a-5p	1.87 ± 0.29*	2.37 ± 0.77
hsa-miR-6819-3p	2.18 ± 0.32*	2.47 ± 0.38

miRNA expression profile differences between ME/CFS patients and matched controls in the replication phase. Data are shown at baseline (T0)and after stress test (T90)by fold difference.

All data are represented as mean ± standard error of the mean. The results were considered significant at *P value < 0.05, and **P value < 0.01.