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. 2020 Nov 12;10:19617. doi: 10.1038/s41598-020-76824-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Signaling pathways profiles in the tumors extracted from animals treated or not with SA or ASA. Western blots displayed are the whole processed strips, as indicated in “Material and methods” and are representative samples of each group and represented in the graphics as mean ± S.E.M. of 8 different animals (n = 8). Panels a and b: AMPK and phospho-AMPK (T172). Panels c and d: ACC and phospho-ACC (S79). Panels e and f: mTOR and phospho-mTOR (S2448). Panels g and h: p70S6K and phospho-p70S6K (T421/S424). Panels i and j: Rictor and phospho-Rictor (T1135). Panels k, l, and m: Akt and phospho-Akt (T308 or S473). Panels n and o: cleaved CAS3 and ß-Actin (Different exposition periods for cleaved CAS3 are presented in supplementary material, Fig. S3). Panels p and q: eNOS and phospho-eNOS (S1177). Panels r and s: LC3B and ß-Actin. Panels t and u: Atg5 and eEF2. *Means P < 0.05 as compared to the control (One-way ANOVA followed by Dunnett post-test).