Table 1:
Sex determination for rat placenta based on Sry (Sry/B2m) values for gDNA and mRNA analyses with all treatment groups combined.a
| Analysis | Sex | Mean ± SEM | Range | Cut-off | p-value |
|---|---|---|---|---|---|
|
| |||||
| gDNA | Male | 1.362 ± 0.1323 | 0.10017 to 6.36962 | >0.05 | <0.0001 |
| gDNA | Female | 1.635 × 10−3 ± 4.868 × 10−4 | N/A (or 0)b to 0.01917 | <0.05 | |
| mRNA | Male | 1.716 ± 0.4422 | 0.07279 to 20.19149 | >0.01 | <0.0001 |
| mRNA | Female | N/A (or 0) | N/A (or 0)b to N/A (or 0)b | <0.01 | |
Descriptive statistics are shown and highlight that no values overlapped between males and females. Hence, cut-offs were determined as indicated. Two-tailed Mann-Whitley tests were used to determine p-values in the male and female comparisons. N=46 and 44 for male and female samples, respectively, for both gDNA and mRNA samples.
A value of N/A (or 0) is derived from the corresponding Sry Cq value of ≥ 40.