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. Author manuscript; available in PMC: 2020 Nov 13.
Published in final edited form as: J Mol Biol Methods. 2020 Jul 5;3(2):107.

Table 1:

Sex determination for rat placenta based on Sry (Sry/B2m) values for gDNA and mRNA analyses with all treatment groups combined.a

Analysis Sex Mean ± SEM Range Cut-off p-value

gDNA Male 1.362 ± 0.1323 0.10017 to 6.36962 >0.05 <0.0001
gDNA Female 1.635 × 10−3 ± 4.868 × 10−4 N/A (or 0)b to 0.01917 <0.05
mRNA Male 1.716 ± 0.4422 0.07279 to 20.19149 >0.01 <0.0001
mRNA Female N/A (or 0) N/A (or 0)b to N/A (or 0)b <0.01
a

Descriptive statistics are shown and highlight that no values overlapped between males and females. Hence, cut-offs were determined as indicated. Two-tailed Mann-Whitley tests were used to determine p-values in the male and female comparisons. N=46 and 44 for male and female samples, respectively, for both gDNA and mRNA samples.

b

A value of N/A (or 0) is derived from the corresponding Sry Cq value of ≥ 40.