Table 4:

Rat placental Sry Cq gDNA and mRNA non-normalized values with all treatment groups combined.^a

Analysis	Sex	Sry Cq (Mean ± SEM)	Sry Cq Range	p-value^b

gDNA	Male	24.56 ± 0.3201	21.92 to 31.08	<0.0001
gDNA	Female	34.52 ± 0.3609^c	30.27 to N/A^a	<0.0001
mRNA	Male	33.92 ± 0.2431	30.32 to 37.17	N/A^a
mRNA	Female	N/A^a	N/A^a	N/A^a

The Bio-Rad CFX program denoted Cq values ≥ 40 as N/A.

The gDNA data were analyzed using a two-tailed unpaired t-test to compare male and female Cq values (excluding four female samples with undetermined Sry Cq values). For mRNA samples, there was no overlap between male and female Cq values, but statistical analysis was not performed because all female samples had undetermined Sry Cq values (≥ 40). Sample sizes are indicated in Table 2.

This particular mean ± SEM calculation excluded the four female samples with undetermined Sry Cq values.