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. 2020 Nov 12;10:19634. doi: 10.1038/s41598-020-76628-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Oocytes after transvaginal sonographically controlled follicle punctures and cumulus removal. The images were taken during initial examination after punctures. GV (a) and MI (b) oocytes are excluded from the egg donation program. Oocytes at MII stage (c) are used in assisted reproductive technologies. Black arrow—the nucleus with a kariosphere, white arrow—a polar body. Scale bar: 50 μm.