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. 2020 Nov 12;3:666. doi: 10.1038/s42003-020-01386-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Gene expression was examined by RT-PCR or quantitative RT-PCR in HEK293T cells following Wnt stimulation or the knockdown of both WNK1 and WNK4. n = 3 biologically independent experiments. Dots indicate individual data. b Gene expression was examined by RT-PCR or quantitative RT-PCR in HEK293T cells following the expression of DVL1 or knockdown of both WNK1 and WNK4. n = 3 biologically independent experiments. Dots indicate individual data. c Gene expression was examined by RT-PCR or quantitative RT-PCR in HEK293T cells following the treatment with Wnt3a stimulation, the expression of DVL1, DVL2 and DVL3, or knockdown of both WNK1 and WNK4. n = 3 biologically independent experiments. Dots indicate individual data. d Gene expression was examined by RT-PCR or quantitative RT-PCR in HEK293T cells following Wnt stimulation of the expression of WNK1 or knockdown of DVL1, DVL2 and DVL3. n = 3 biologically independent experiments. Dots indicate individual data. e Gene expression was examined by RT-PCR or quantitative RT-PCR in HEK293T cells following the expression of β-Catenin or knockdown of both WNK1 and WNK4. n = 3 biologically independent experiments. Dots indicate individual data. f Gene expression was examined by RT-PCR or quantitative RT-PCR in HEK293T cells following Wnt stimulation, the expression of WNK1 or knockdown of β-Catenin. n = 3 biologically independent experiments. Dots indicate individual data. g Western blot analysis of endogenous protein expression following Wnt stimulation, the knockdown of both WNK1 and WNK4, or MG132 (10 μM, 6 h) treatment in HEK293T cells. n = 3 biologically independent experiments. Dots indicate individual data. h Western blot analysis of ubiquitinated β-Catenin under the expression of WNK1 and WNK4 in HEK293T cells. Values and error bars express mean ± standard deviation (SD). * indicates p < 0.05 and ** indicates p < 0.005. p value was calculated by Bonferroni test.