KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Polyclonal antibody against Lamprey CFTR	This paper	New England Peptide (Boston MA):Item # 3813
Bacterial and Virus Strains
Biological Samples
Chemicals, Peptides, and Recombinant Proteins
Adenosine Triphosphate	G. Cui et al, 2013.	SigmaAldrich(St. Louis, MO): CAS# 56-65-5
Protein Kinase A	G. Cui et al, 2016.	Promega (Madison, WI): CAS# 142008-29-5
Forskolin	D. Infield et al, 2016	SigmaAldrich (St. Louis, MO): CAS# 66575-29-9
GlyH-101	G. Cui et al, 2014	EMD Millipore (Burlington, MA): CAS# 328541-79-3
Gliblenclamide	G. Cui et al, 2012	SigmaAldrich (St. Louis, MO): CAS#:10238-21-8
CFTRINH-172	D. Infield et al, 2016	Cayman Chem (Ann Arbor, MI): CAS# 307510-92-5
VX-770	G. Cui et al, 2016.	Selleckchem (Houston, TX): CAS# 873054-44-5
Lp-CFTR peptide	This paper	New England Peptide (Boston, MA): TLRHSIKHISLQE, amino acids 1441-1453
Critical Commercial Assays
Deposited Data
Genbank (Lp-CFTR sequences)	This paper	MN216026 and MN216027
Experimental Models: Cell Lines
Experimental Models: Organisms/Strains
Oocytes	Xenopus1 (Dexter, MI)	Class V Xenopus laevis oocytes
Petromyzon marinus	Hammond Bay or Luddington Bio. Station	Larval M2 lamprey
Oligonucleotides
Primers for cloning lamprey CFTR 5’ATGCAAGCTTGGATCAGCCGCATGAGTTCAT 3’	This paper	Integrated DNA Technologies
Primers for RT-qPCR for lamprey CFTR 5’ACAGAGAAATCTCGCAGTCAGAAAACCGCCCCCA CCTGCTCACCGCGATTCGGCGGTGCTTCCT3’	This paper	Integrated DNA Technologies
Recombinant DNA
hCFTRcDNA	Provided by D. Gadsby	G. Cui et al, 2016.
Lp-CFTR cDNA	This paper
Software and Algorithms
MODELLER	Sali, A., Blundell, T.L., 1993	https://salilab.org/modeller/
PROCHECK	Laskowski, R.A. et al., 1993	https://www.ebi.ac.uk/thornton-srv/software/PROCHECK/
MEGA7	Kumar, S et al., 2016.	https://megasoftware.net/
Other
Homology model of lamprey CFTR (closed and nearly-open state)	This paper	Supplemental Figure 1