REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
Polyclonal antibody against Lamprey CFTR | This paper | New England Peptide (Boston MA):Item # 3813 |
Bacterial and Virus Strains | ||
Biological Samples | ||
Chemicals, Peptides, and Recombinant Proteins | ||
Adenosine Triphosphate | G. Cui et al, 2013. | SigmaAldrich(St. Louis, MO): CAS# 56-65-5 |
Protein Kinase A | G. Cui et al, 2016. | Promega (Madison, WI): CAS# 142008-29-5 |
Forskolin | D. Infield et al, 2016 | SigmaAldrich (St. Louis, MO): CAS# 66575-29-9 |
GlyH-101 | G. Cui et al, 2014 | EMD Millipore (Burlington, MA): CAS# 328541-79-3 |
Gliblenclamide | G. Cui et al, 2012 | SigmaAldrich (St. Louis, MO): CAS#:10238-21-8 |
CFTRINH-172 | D. Infield et al, 2016 | Cayman Chem (Ann Arbor, MI): CAS# 307510-92-5 |
VX-770 | G. Cui et al, 2016. | Selleckchem (Houston, TX): CAS# 873054-44-5 |
Lp-CFTR peptide | This paper | New England Peptide (Boston, MA): TLRHSIKHISLQE, amino acids 1441-1453 |
Critical Commercial Assays | ||
Deposited Data | ||
Genbank (Lp-CFTR sequences) | This paper | MN216026 and MN216027 |
Experimental Models: Cell Lines | ||
Experimental Models: Organisms/Strains | ||
Oocytes | Xenopus1 (Dexter, MI) | Class V Xenopus laevis oocytes |
Petromyzon marinus | Hammond Bay or Luddington Bio. Station | Larval M2 lamprey |
Oligonucleotides | ||
Primers for cloning lamprey CFTR 5’ATGCAAGCTTGGATCAGCCGCATGAGTTCAT 3’ |
This paper | Integrated DNA Technologies |
Primers for RT-qPCR for lamprey CFTR 5’ACAGAGAAATCTCGCAGTCAGAAAACCGCCCCCA CCTGCTCACCGCGATTCGGCGGTGCTTCCT3’ |
This paper | Integrated DNA Technologies |
Recombinant DNA | ||
hCFTRcDNA | Provided by D. Gadsby | G. Cui et al, 2016. |
Lp-CFTR cDNA | This paper | |
Software and Algorithms | ||
MODELLER | Sali, A., Blundell, T.L., 1993 | https://salilab.org/modeller/ |
PROCHECK | Laskowski, R.A. et al., 1993 | https://www.ebi.ac.uk/thornton-srv/software/PROCHECK/ |
MEGA7 | Kumar, S et al., 2016. | https://megasoftware.net/ |
Other | ||
Homology model of lamprey CFTR (closed and nearly-open state) | This paper | Supplemental Figure 1 |