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The Amino acid sequence and disulfide bonds of TPN-RQ are illustrated. Each amino acid was classified into five groups and shown in different colors in order of reduction in ROMK blocking activity when each amino acid was changed to Ala (red >80%; orange, 60–80%; yellow, 40–60%; green, 20–40%; blue, <20% at 1 μM) (see text and Table 1).
