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. 2020 Nov 3;16(11):e1009188. doi: 10.1371/journal.pgen.1009188

Fig 7. The LET-653 ZPc domain binds aggregates in vitro.

Fig 7

A-G) Single confocal slices of freshly thawed cell media. A-B) Aggregates were non-fluorescent in EV- or SfGFP- transfected cell media. C) Aggregates were fluorescent in SfGFP::LET-653(ZP)-transfected media. D) SfGFP::LET-653(ZPn)-transfected media resembled negative controls; E) SfGFP::LET-653(ZPc) transfected cell media contained aggregates of high fluorescence intensity. F-G) Aggregates in media containing LET-653(ZP)::SfGFP and LET-653(ZP, AYAA)::SfGFP were similarly fluorescent. H) Quantification of aggregate fluorescence intensity. ***p<0.0001; two-tailed Mann-Whitney U test. Error bars; standard error. All experiments were performed on media from transfection 2.