Fig 8. LET-653-bound aggregates stain with dyes and are not heat-labile.

A-B) Aggregates stained with the lipid dye Nile Red regardless of whether LET-653(ZPc) was present. A) LET-653(ZPc) and Nile Red co-labelled aggregates. B) When LET-653(ZPc) was absent, aggregates, which were rare, still stained strongly with Nile Red. C) DIC images of LET-653(ZPc) aggregates. Aggregates were plentiful in freshly thawed media and remained after media was incubated for 15 minutes at 50 °C. D) Quantification of aggregate number. No significant differences were seen between any groups, two-tailed Mann Whitney U test. Error bars; standard error.