Table 2. RT-PCR primers of 8 most significant hub genes and reference gene, HPRT1.

Gene name	Full name	Primer sequence(5'→3')	Tm(°C)	Product(bp)
CDC20	cell division cycle 20, aliases: CDC20A	Forward: AGTTCGCGTTCGAGAGT	55.7	195
		Reverse: GAACCTTGGAACTGGAT	50
BUB1	budding uninhibited by benzimidazoles-1, aliases: BUB1A	Forward: TATAGCAGGCTGATTGGGCT	58.5	107
		Reverse: TGGCTTAAACAGGTCAGTGT	57
TOP2A	Topoisomerase II alpha	Forward: GTGTCACCATTGCAGCCTGT	61	152
		Reverse: GAACCAATGTAGGTGTCTGG	56
UBE2C	ubiquitin conjugating enzyme E2 C	Forward: CTCATGGTATATGAAGACCT	51	132
		Reverse: GCATATGTTACCCTGGGTGT	57
BUB1B	BUB1 mitotic checkpoint serine/threonine kinase B	Forward: TGCTCTGAGTGAAGCCATGT	59	99
		Reverse: TGAAGCGTGGACATGATCCG	60
RRM2	ribonucleotide reductase regulatory subunit M2	Forward: GGGAATCCCTGAAACCCGAG	60	70
		Reverse: CCATCGCTTGCTGCAAAGAA	59.7
CCNA2	cyclin A2	Forward: GGATGGTAGTTTTGAGTCACCAC	59	202
		Reverse: CACGAGGATAGCTCTCATACTGT	59
MAD2L1	mitotic arrest deficient 2 like 1	Forward: ACGGTGACATTTCTGCCACT	60	105
		Reverse: TGGTCCCGACTCTTCCCATT	60.5
HPRT1	hypoxanthine phosphoribosyl-transferase 1	Forward: GGACTAATTATGGACAGGACTG	55	195
		Reverse: GCTCTTCAGTCTGATAAAATCTAC	55