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. 2020 Oct 20;223(5):765–774. doi: 10.1093/infdis/jiaa658

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© The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 1. — LC480II plot amplifications and standard curves of the singleplex and multiplex assays. Each assay was tested using related standard ranging from 10⁷ to 10 copies/μL. Polymerase chain reaction curve data are represented by relative fluorescence plotted against cycle threshold (Ct) values. Standard curves were generated from the Ct values obtained against known concentrations. Slope of the regression curve, the coefficient of determination (R²), and efficiency (E) are indicated for each assay.