Table 1. Candidate reference genes of Neonectria ditissima, primers and their efficiencies when used in qRT-PCR.
| Gene name | Gene ID and accession number | Abbreviation | Forward Primer | Reverse Primer | Size (bp) | Efficiency (E) | R2 value* |
|---|---|---|---|---|---|---|---|
| Actin | Ndactin MT040710 | actin | CTCTGTTCCAGCCCTCAGTC | TCGGACATCGACATCACACT | 92 | 1.97 | 0.9940 |
| Beta-tubulin | Ndbtub MT040711 | Btub | TGGAAGTCAAGCACGATGAG | ATGTGCCCCACATCTCTTTC | 90 | 2.07 | 0.9959 |
| Myo-inositol-1-phosphate synthase | Ndmips MT040712 | mips | TGTTCAACATCTGCGAGGAC | GCCTTCCACTGGATACGAGA | 94 | 1.96 | 0.9895 |
| Elongation factor thermo unstable | NdEfTu MT040713 | EfTu | GATGCCAGTGGATCTTCACC | TGAGGCTTTGTCGAGTGTTG | 82 | 1.84 | 0.9813 |
| 18s ribosomal RNA adenine methylase transferase | Nd18sAMT MT040714 | 18sAMT | TCCGCAAGAACAAGACACTG | ACCATCCTCGATGTCCATGT | 167 | 2.01 | 0.9883 |
| 40S ribosomal protein subunit S8 | NdS8 MT040715 | S8 | CTCTTACCACCCCTCGAACA | TTCTTCACGTCCTCCTCGAC | 183 | 1.91 | 0.9879 |
| 40S ribosomal protein subunit S27a | NdS27a MT040716 | S27a | TCGACAACGTCAAGTCCAAG | CTTCTTGGGGGTGGTGTAGA | 203 | Not tested | Not tested |
| Ubiquitin-conjugating enzyme | NdE2 MT040717 | E2 | CTCCGACATGGAGAGGAGAG | GAGAGGCCCAGATACCCTTC | 235 | Not tested | Not tested |
* Coefficient of correlation.