Table 2. Quality evaluation for degradome sequencing data.
Sample | 9311-2x (number) | 9311-2x (ratio) | 9311-4x (number) | 9311-4x (ratio) | Total (number) | Total (ratio) |
---|---|---|---|---|---|---|
Raw reads | 22150505 | / | 20490420 | / | 42640925 | / |
Reads < 15 nt after removing 3 adapters | 60903 | 0.27% | 60601 | 0.30% | 121504 | 0.28% |
Mappable reads | 22089602 | 99.73% | 20429819 | 99.70% | 42519421 | 99.72% |
Unique raw reads | 5275288 | / | 4704761 | / | 8797333 | / |
Unique reads<15nt after removing 3 adapters | 25777 | 0.49% | 24106 | 0.51% | 43977 | 0.50% |
Unique mappable reads | 5249511 | 99.51% | 4680655 | 99.49% | 8753356 | 99.50% |
Transcript mapped reads | 2579573 | 11.65% | 5143435 | 25.10% | 7723008 | 18.11% |
Unique transcript mapped reads | 395259 | 7.49% | 686180 | 14.58% | 950717 | 10.81% |
Number of input transcripts | 43891 | / | 43891 | / | 43891 | / |
Number of covered transcripts | 17679 | 40.28% | 19843 | 45.21% | 21106 | 48.09% |