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. Author manuscript; available in PMC: 2021 Jun 21.
Published in final edited form as: Biomater Sci. 2020 May 26;8(12):3522–3535. doi: 10.1039/d0bm00521e

Figure 4. Both Coil29 and Q11 nanofibers induced low levels of inflammatory cytokines and inflammatory cell influx, relative to both Alum and SAS adjuvanted groups.

Figure 4.

Cytokine responses and cellular responses in the lavage fluid were analyzed for C57BL/6 mice intraperitoneally immunized with either nanofibers or peptide/adjuvant formulations. All values reported are mean ± SEM. (A) Cytokine secretion levels were determined using cytokine ELISA 12 hours after i.p. immunization. Abbreviation: MCP-1 (monocyte chemotactic protein-1), G-CSF (granulocyte colony-stimulating factor), TNF (tumor necrosis factor), IL (interleukin). (B) Immune cell populations in lavage fluid were analyzed 1 hour and 12 hours after immunization using flow cytometry. For both (A) and (B): * p<0.05, ** p<0.01, *** p<0.001 **** p<0.0001 by two-way ANOVA with Tukey post-hoc test.