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. Author manuscript; available in PMC: 2021 Jun 21.
Published in final edited form as: Biomater Sci. 2020 May 26;8(12):3522–3535. doi: 10.1039/d0bm00521e

Figure 6. Coil29 nanofibers induced greater numbers of follicular T helper (Tfh) cells than Q11 nanofibers.

Figure 6.

(A) Draining lymph nodes were harvested 7 days after immunizations with either pOVA-Coil29 or pOVA-Q11 nanofibers. Tfh (CXCR5+PD-1+) were counted among CD44+CD4+ T cells. Representative flow cytometry plots (left) and Tfh cell frequency (right) are shown (n = 4, p-value calculated with unpaired t-test). (B) Coil29 nanofibers induced platform-specific Tfh cell responses. Lymphocytes from draining lymph nodes of immunized mice were split into three groups (unstimulated, pOVA-stimulated, Coil29 stimulated). CD25+OX40+ cells were counted by flow cytometry 18 hours after stimulation treatments. Representative flow plots (left) and antigen-specific Tfh cell frequency (right) are shown. P value was calculated with multiple t test using Holm-Sidak method for multiple comparison correction.