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. Author manuscript; available in PMC: 2021 Jun 21.
Published in final edited form as: Biomater Sci. 2020 May 26;8(12):3522–3535. doi: 10.1039/d0bm00521e

Figure 7. Coil29 nanofibers induced strong long-term E214-specific IgG humoral responses and promoted higher antigen-specific memory B cell frequency when coassembled with additional (0.01 mmol) PADRE T cell epitopes.

Figure 7.

Groups of C57BL/6 mice (n=5) were immunized s.c. either with 134 μg of E214 peptides (0.067 mmol) delivered with Alum or SAS adjuvant, with E214-Coil29 (E214-C), or E214-Q11 (E214-Q) nanofibers carrying equivalent amounts of E214 peptides, with or without co-assembled PADRE-Coil29 or PADRE-Q11 (PAD-C or PAD-Q, respectively) followed by booster injections on week 4. All values shown are mean ± SEM. (A) Total serum anti-E214 IgG titers measured by ELISA (* p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001 compared to E214/Alum group by two-way ANOVA with Dunnett post-hoc test). (B) Frequency of E214-specific memory B cells among memory B cells from immunized mice 30 weeks after primary immunizations. *p<0.05 compared to E214-Q group by ordinary one-way ANOVA with Dunnett post-hoc test.