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. 2020 Nov 2;9:e60151. doi: 10.7554/eLife.60151

Figure 4. eIF4E S209 promotes Myc-driven oncogenesis in mice and human.

(A) Representative IF of indicated markers in polyps and adjacent ‘normal’ crypts in ApcMin/+ mice. Scale bar: 100 µm. (B) Representative images of polyps and (C) quantification in the small intestine of 3-month-old ApcMin/+ (4E+/+) (n = 5), ApcMin/+;4EKI/+ (n = 8), and ApcMin/+;4EKI/KI (n = 4) mice. ++p<0.01, +++p<0.001 (One-Way ANOVA with TUKEY Post-hoc test). (D) Representative IF of indicated markers and (E) quantitation in the polyps in indicated mice. Scale bar: 100 µM. (F) Myc mRNA levels in the polyps from indicated mice. (G) p-4E (S209) and total eIF4E in human adenomas and normal colon analyzed by western blotting. Actin is a loading control.

Figure 4.

Figure 4—figure supplement 1. eIF4E S209 promotes polyposis in ApcMin/+ mice.

Figure 4—figure supplement 1.

(A) Representative images of 4EKI genotypes in ApcMin/+ mice. (B) Tumor burden in the proximal (PI), middle (MI) and distal intestine (DI), and C, colon. Values represent mean+s.d. +p<0.05, ++p<0.001 (One-Way ANOVA with TUKEY Post-hoc test). (C) Enlarged p-4E and KI-67 IF in the ‘normal’ crypts of the indicated mice. Scale bar: 50 µM. (D) Representative Ki-67 IF in ‘normal’ intestinal crypts in the indicated mice. Scale bar: 100 µm, and (E) Quantification. (F) Indicated transcripts in the mucosa of 6-week-old (pre-cancerous) ApcMin/+, and ApcMin/+, 4EKI were analyzed by RT-PCR and normalized to that in ApcMin/+ (Siegel et al., 2019). N = 3. Values are mean+s.d. *p<0.05, **p<0.01 (Student’s t-test, two tailed). WT vs. KI. (G) Representative IF of indicated markers in the polyps and H and E image of polyps. Scale bar: 100 µM, and (H) quantitation.