Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo-sapiens) |
HEK293T | ATCC | CRL-3216 RRID:CVCL_0063 |
|
| Cell line (Homo-sapiens) |
TZM-bl | NIH AIDS Reagent Resource | 8129–442 RRID:CVCL_B478 |
|
| Strain, strain background (Influenza A Virus) | A/PR/8/34 (H1N1) | Charles River Laboratories | 10100781 | Clarified allantoic fluid |
| Recombinant DNA reagent | pNL4-3 (plasmid) |
NIH AIDS Reagent Resource | 114 | |
| Recombinant DNA reagent | pNL4-3ΔEnv (plasmid) |
Eric O. Freed | ||
| Recombinant DNA reagent | pMD2.G (VSV-G) (plasmid) |
Addgene | 12259 RRID:Addgene_12259 |
|
| Recombinant DNA reagent | pCMV4-BlaM-Vpr (plasmid) |
Addgene | 21950 RRID:Addgene_21950 |
|
| Recombinant DNA reagent | pReceiver-YFP-IFITM3 and mutants (plasmid) |
This paper | YFP fused to IFITM3 at amino terminus | |
| Recombinant DNA reagent | pReceiver-mCherry-IFITM3 and mutants (plasmid) |
This paper | mCherry fused to IFITM3 at amino terminus | |
| Recombinant DNA reagent | pReceiver-PRRT2-YFP and mutants (plasmid) |
This paper | YFP fused to PRRT2 at carboxy terminus | |
| Recombinant DNA reagent | pReceiver-PRRT2-mCherry and mutants (plasmid) |
This paper | mCherry fused to PRRT2 at carboxy terminus | |
| Recombinant DNA reagent | pQCXIP-FLAG-IFITM3 and mutants (plasmid) |
Compton et al., 2014 and this paper | FLAG fused to IFITM3 at amino terminus | |
| Recombinant DNA reagent |
EEA1-GFP (plasmid) |
Addgene | 42307 RRID:Addgene_42307 |
|
| Commercial assay or kit | LiveBLazer FRET-B/G Loading Kit with CCF2-AM | Thermo Fisher | K1032 | |
| Chemicalcompound, drug | Amphotericin B | Sigma | C4951 | |
| Chemical compound, drug | FliptR | Spirochrome | CY-SC020 | |
| Chemical compound, drug | Laurdan | Invitrogen | D250 | |
| Chemical compound, drug | Cholesterol, water-soluble | Sigma | A2942 | |
| Chemical compound, drug | Methyl-beta-cyclo-dextrin | Sigma | C4555 | |
| Antibody | Anti-IAV NP mouse monoclonal | Abcam | AA5H | 1:500 (flow) |
| Antibody | Anti-p24 CA mouse monoclonal | NIH AIDS Reagent Resource | 3537 | 1:1000 (WB) |
| Antibody | Anti-p24 CA [KC57-FITC] mouse monoclonal |
BD | CO6604665 | 1:500 (flow) |
| Antibody | Anti-CD63 [MX-49.129.5] mouse monoclonal |
Santa Cruz Biotechnology | sc-5275 | 1:400 (IF) |
| Antibody | Anti-FLAG [M2] mouse monoclonal |
Sigma | F1804 | 1:1000 (WB) 1:500 (flow) |
| Antibody | Anti-IFITM3 [EPR5242] rabbit monoclonal |
Abcam | ab109429 | 1:1000 (WB) 1:200 (IF) |
| Antibody | Anti-Env gp120b sheep polyclonal | NIH AIDS Reagent Resource | 288 | 1:1000 (WB) |
| Antibody | Anti-Env gp41 [2F5] human monoclonal |
NIH AIDS Reagent Resource | 1475 | 1:1000 (WB) |
| Antibody | Anti-Actin [C4] mouse monoclonal |
Santa Cruz Biotechnology | sc-47778 | 1:1000 (WB) |
| Antibody | Anti-c-Myc rabbit monoclonal | Sigma | C3956 | 1:1000 (WB) |